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miRNA in Human Disease: From Biomarkers to Therapeutics explores the critical role of microRNAs (miRNAs) in the development and progression of various diseases, emphasizing their significance as biomarkers and therapeutic targets. This book provides a comprehensive overview of the biogenesis, function, and regulation of miRNAs, offering insights into how these small noncoding RNA molecules influence gene expression and contribute to disease development and progression. The book covers a wide range of topics, including traditional and advanced methods for miRNA extraction, the role of miRNAs in infectious diseases, cancer, neurological disorders, cardiovascular disease, hemoglobinopathies, pregnancy complications, and metabolic disorders. It also discusses the latest research on miRNA-based therapies, including the design and delivery of miRNA mimics and inhibitors, and their potential applications in personalized medicine. With a strong focus on practical implementation, the book includes detailed case studies and real-world examples that demonstrate the clinical relevance of miRNA research. It addresses the challenges and future directions in the field, providing a roadmap for researchers, clinicians, and industry professionals to harness the potential of miRNAs for disease diagnosis, treatment, and prevention. This book is an essential resource for research scientists in molecular biology, genetics, and biochemistry, as well as clinicians and medical professionals such as oncologists, neurologists, and pathologists. It also serves as a valuable reference for graduate students, postdoctoral researchers, academics, and industry professionals working in biotechnology, pharmaceuticals, and diagnostics.